Novel Species of Oculatellaceae (Oculatellales, Cyanobacteria) from Yunnan in China, Based on the Polyphasic Approach
Journal Article
Overview
abstract
Oculatellaceae is a family of cyanobacteria with orange spots in the apical cells and has a wide distribution in various living environments. The species of this family are widely distributed but relatively few in number. In order to enrich our knowledge of the species diversity of cyanobacteria in China, and further achieve the monophyletic development of modern cyanobacteria classification systems, we studied two algal strains, designated as SXACC0114 and SXACC0117, isolated from China and subjected to taxonomic studies using a multiphase approach. The colony of the strain SXACC0114 is bright blue-green in color and does not form a biofilm. The trichomes are yellow-green to bright blue-green. For the strain SXACC0117, no false branching is observed. It has wider filaments and more distinct sheaths, and lacks swollen cells. Based on 16S rRNA gene phylogenetic analysis, the results showed that these two algal strains clustered in Albertania and Tildeniella evolutionary branches, respectively, with high bootstrap support. In addition, the secondary structures, which are constructed based on the internal transcription spacer (ITS) of 16S-23S rRNA, exhibit differences, and the algal strain has unique D1-D1สน, Box-B, and V3 helix structures. These results support the establishment of two new species, described as Albertania yunnanense sp. nov. and Tildeniella yunnanense sp. nov. The discovery of these new species provides a scientific basis for the development and utilization of algae.
